水稻光溫敏雄性核不育系廣占63S的不育基因來源于光敏核不育系農(nóng)墾58S，其育性轉(zhuǎn)換表現(xiàn)為溫敏型，是迄今兩系雜交水稻中應(yīng)用最廣泛的秈型光溫敏核不育系之一。對廣占63S/旱1587組合的F2分離群體進行不育基因的遺傳分析，結(jié)果表明廣占63S的不育性受1對隱性不育基因控制。利用SSR和InDel分子標(biāo)記技術(shù)，結(jié)合BSA法，以廣占63S與旱1587組合的F2分離群體為材料，將不育基因（暫命名為PTGMS2-1）定位于第2染色體標(biāo)記S2-4與S2-27之間物理距離390kb的區(qū)間內(nèi)，與兩標(biāo)記間的遺傳距離分別為0.5和1.1cM，與標(biāo)記S2-24共分離。
Guangzhan 63S,a photoperiod-thermo-sensitive genic male sterile (PTGMS) line, was one of the most widely used PTGMS lines in two-line indica hybrid rice breeding system in China. Genetic analysis indicated that the sterility of Guangzhan 63S was controlled by a single recessive gene using the F2 population derived from a cross between the Guangzhan 63S and Han 1587. Molecular mapping was performed on the F2 mapping population using SSR and InDel markers and bulked-segregant analysis(BSA). The PTGMS2-1 was restricted to a 390 kb region on chromosome 2 between the markers S2-4 and S2-27, at genetic distances of 0.5 cM and 1.1 cM, respectively, and co-segregated with S2-24.